DNA SEQUENCE ANALYSIS

DNA sequence analysis: translation

Determination of the nucleotide sequence of a length of DNA. Typically, this is performed by cloning the DNA of interest, so that enough can be prepared to allow the sequence to be determined, usually by the Sanger dideoxy sequencing method or the Maxam-Gilbert method. The resulting reactions are then run on a large sequencing gel, capable of resolving single nucleotide differences in chain length. Recently, PCR-based methods have obviated the need to clone the DNA under some conditions, and automated DNA sequencing has become widely available.