BOYDEN CHAMBER

Boyden chamber: translation

Simple chamber used to test for chemotaxis, especially of leucocytes. Consists of two compartments separated by a millipore filter (3-8 m m pore size) ; chemotactic factor is placed in one compartment and the gradient develops across the thickness of the filter (ca 150 m m). Cell movement into the filter is measured after an incubation period less than the time taken for the gradient to decay. See also checkerboard assay.